register_localizations#
Invoke#
Inside the folder with your input data, run:
pyhim -C register_localizations
Inputs#
Name shape |
Quantity |
Mandatory |
Description |
---|---|---|---|
parameters.json |
1 |
Yes |
Parameter file. |
Outputs#
Name shape |
Quantity |
Description |
---|---|---|
Relevant options#
Parameters to run this script will be read from the buildsPWDmatrix
field of parameters.json
.
"toleranceDrift": 1 # tolerance drift in pixels. Above this value localizations will not be locally registered
outputs images:
_registered_barcode_stats.png
_registered_barcode_localizations_ROI*.png
statistics of registration |
localization map |
---|---|
Alignment accuracy
This provides a map of all barcode localizations in an ROI, colorcoded by the accuracy of localization. Colorbar scale is in pixels.