register_localizations#

Invoke#

Inside the folder with your input data, run:

pyhim -C register_localizations

Inputs#

Name shape

Quantity

Mandatory

Description

parameters.json

1

Yes

Parameter file.

Outputs#

Name shape

Quantity

Description

Relevant options#

Parameters to run this script will be read from the buildsPWDmatrix field of parameters.json.

"toleranceDrift": 1 # tolerance drift in pixels. Above this value localizations will not be locally registered

outputs images:

  • _registered_barcode_stats.png

  • _registered_barcode_localizations_ROI*.png

statistics of registration

localization map

image-20220210221852444

image-20220210221942291

image-20220210222028835

image-20220210222006297

Alignment accuracy

This provides a map of all barcode localizations in an ROI, colorcoded by the accuracy of localization. Colorbar scale is in pixels.