mask_cellpose#

This script is provided for the user to be able to segment nuclei or other objects (e.g. mask images) using cellpose.

For more information, follow this tutorial: Running cellpose to create 3D masks for pyHiM

Invoke#

To process

$ mask_cellpose.py --input my_image.tif 

Inputs#

Name shape

Quantity

Mandatory

Description

<image_name>.tif

1

Yes

3D image in tif format.

parameters.json

1

Yes

Parameter file.

register_global/data/shifts.json

1

Yes

Output JSON file of register_global with the shift values.

Outputs#

Name shape

Quantity

Description

mask_3d/data/*_3Dmasks.npy

1

NUMPY 3D array. The output labeled image.

mask_2d/data/*_Masks.npy

1

NUMPY 2D array. The output labeled image, projected by maximum.