mask_3d#

Segments masks in 3D

Invoke#

Inside the folder with your input data, run:

pyhim -C mask_3d

segmentation

Inputs#

Name shape

Quantity

Mandatory

Description

parameters.json

1

Yes

Parameter file.

<image_name>.tif

2..n

Yes

3D images

Outputs#

Name shape

Quantity

Description

*_3Dmasks.npy

2..n

A 3D mask segmentation produces two outputs saved in the segmentedObjects folder:

scan_002_mask0_002_ROI_converted_decon_ch01.tif_3Dmasks.png
scan_002_mask0_002_ROI_converted_decon_ch01._3Dmasks.npy

The PNG file is a representation of the raw image and the segmented objects.

The NPY file is a 3D labeled numpy array containing the segmented objects. The file name is constructed using the original root filename with the tag _3DMasks.

Warning: This mode operates in 3D, therefore the Startdist network provided must be in 3D.

Relevant options#

Most of the parameters are shared with mask_2d, except for the following:

Name

Option

Description

stardist_basename

Folder containing 2D and 3D AI models

stardist_network3D

Name of the 3D network